摘要
2016年，Rasool等人首次指出Ti3C2材料具有超强的抗菌性能，使得二维纳米材料作为最有前景的抗菌纳米材料之一成为人们关注的焦点。Gogotsi的研究表明，MXene材料具有超薄层状结构，其抗菌活性明显优于氧化石墨烯材料。本试验以二维纳米Ti3C2材料为原料，通过银氨溶液添加的方法制备了Ti3C2-Ag配合物，测试了该配合物对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抗菌活性，分析了该配合物的抗菌机理。研究发现，该配合物对大肠杆菌的抗菌活性可达98.38%。对大肠杆菌的抑菌率为99.08%，对金黄色葡萄球菌的抑菌率为99.08%，抑菌率与银含量、作用时间呈正相关。其抗菌机制主要包括：（1）纳米片对细胞膜的物理切割；（2）银纳米粒子刺激氧化应激反应等。以上结果表明Ti3C2-Ag是一种很有前景的抗菌材料。
关键词： Ti3 C2 -Ag配合物的制备；抗菌作用；机理
Abstract
In 2016, Rasool et al. first demonstrated the exceptional antibacterial properties of Ti3C2, making two-dimensional nanomaterials a focal point of attention as one of the most promising antibacterial nanomaterials. Gogotsi et al. have shown that MXene materials, with their ultrathin layered structure, exhibit significantly superior antibacterial activity compared to graphene oxide. In this study, a Ti3C2-Ag complex was prepared using two-dimensional nano-Ti3C2 as a raw material by adding a silver ammonia solution. The antibacterial activity of the complex against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was tested, and the antibacterial mechanism of the complex was analyzed. The complex exhibited an antibacterial activity of 98.38% against E. coli and 99.08% against S. aureus, respectively. The inhibition rates were positively correlated with the silver content and exposure time. Its antibacterial mechanism mainly includes: (1) physical cutting of cell membrane by nanosheets; (2) stimulation of oxidative stress response by silver nanoparticles, etc. The above results indicate that Ti3C2-Ag is a promising antibacterial material.
Key words： Ti3C2 -Ag complexes preparation; Antibacterial; Mechanism
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